Serotonin-containing projections to the thalamus in the rat revealed by a horseradish peroxidase and peroxidase antiperoxidase double-staining technique
Identifieur interne : 001792 ( Main/Exploration ); précédent : 001791; suivant : 001793Serotonin-containing projections to the thalamus in the rat revealed by a horseradish peroxidase and peroxidase antiperoxidase double-staining technique
Auteurs : Adriana Consolazione [Italie] ; John V. Priestley [Royaume-Uni] ; A. Claudio Cuello [Royaume-Uni]Source :
- Brain Research [ 0006-8993 ] ; 1984.
English descriptors
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Abstract
Abstract: The retrograde transport of horseradish peroxidase (HRP) has been used in combination with peroxidase antiperoxidase (PAP) immunocytochemistry in order to investigate serotonin-containing projections to the thalamus of the rat. Sections were histochemically stained to reveal retrogradely transported HRP and then PAP immunostained using a monoclonal anti-serotonin (5-HT) antibody. Following HRP injections into the ventral thalamus, retrogradely labelled cells were observed in a number of sites in the brainstem and including areas known to be rich in 5-HT-containing neurons. At rostral levels of the dorsal raphe nucleus, retrogradely labelled cells were observed both on the midline and in a distinct lateral group extending diffusely into the periaqueductal gray (PAG). In both of these areas many 5-HT-immunoreactive HRP retrogradely labelled neurons were observed. However, except for the most rostral levels of the dorsal raphe nucleus, such double-labelled cells represented only a small proportion of the total population of 5-HT-immunoreactive neurons. In the lateral group, the retrograde labelling was mainly unilateral to the injection site but some contralateral labelling was also seen. At caudal levels of the dorsal raphe nucleus, retrogradely labelled cells were observed predominantly in the lateral group. At the level of the dorsolateral tegmental nucleus, few 5-HT of 5-HT/HRP labelled cells were observed in the lateral group, although HRP retrogradely labelled neurons were present. Double-stained cells were detected also in the medial raphe nucleus (corresponding to the B8 cell group according to the nomenclature of Dahlström and Fuxe13), among the fibres of the medial lemniscus (B9), and in nucleus raphe pontis (B5).
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DOI: 10.1016/0006-8993(84)90113-6
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Priestley</name><affiliation wicri:level="3"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Correspondence: J.V. Priestley, Neuroanatomy-Neuropharmacology Group, Departments of Pharmacology and Human Anatomy, South Parks Road, Oxford</wicri:regionArea><placeName><settlement type="city">Oxford</settlement><region type="country">Angleterre</region><region type="comté" nuts="2">Oxfordshire</region></placeName></affiliation><affiliation wicri:level="3"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Neuroanatomy-Neuropharmacology Group, Departments of Pharmacology and Human Anatomy, Oxford</wicri:regionArea><placeName><settlement type="city">Oxford</settlement><region type="country">Angleterre</region><region type="comté" nuts="2">Oxfordshire</region></placeName></affiliation></author><author><name sortKey="Cuello, A Claudio" sort="Cuello, A Claudio" uniqKey="Cuello A" first="A. Claudio" last="Cuello">A. Claudio Cuello</name><affiliation wicri:level="3"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Neuroanatomy-Neuropharmacology Group, Departments of Pharmacology and Human Anatomy, Oxford</wicri:regionArea><placeName><settlement type="city">Oxford</settlement><region type="country">Angleterre</region><region type="comté" nuts="2">Oxfordshire</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Brain Research</title><title level="j" type="abbrev">BRES</title><idno type="ISSN">0006-8993</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1984">1984</date><biblScope unit="volume">322</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="233">233</biblScope><biblScope unit="page" to="243">243</biblScope></imprint><idno type="ISSN">0006-8993</idno></series><idno type="istex">2D74DE6A93B6AF59AEC3DF67C076FD8F1E65B7F8</idno><idno type="DOI">10.1016/0006-8993(84)90113-6</idno><idno type="PII">0006-8993(84)90113-6</idno><idno type="ArticleID">84901136</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0006-8993</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>5-HT</term><term>HRP</term><term>immunocytochemistry</term><term>raphe</term><term>thalamus</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The retrograde transport of horseradish peroxidase (HRP) has been used in combination with peroxidase antiperoxidase (PAP) immunocytochemistry in order to investigate serotonin-containing projections to the thalamus of the rat. Sections were histochemically stained to reveal retrogradely transported HRP and then PAP immunostained using a monoclonal anti-serotonin (5-HT) antibody. Following HRP injections into the ventral thalamus, retrogradely labelled cells were observed in a number of sites in the brainstem and including areas known to be rich in 5-HT-containing neurons. At rostral levels of the dorsal raphe nucleus, retrogradely labelled cells were observed both on the midline and in a distinct lateral group extending diffusely into the periaqueductal gray (PAG). In both of these areas many 5-HT-immunoreactive HRP retrogradely labelled neurons were observed. However, except for the most rostral levels of the dorsal raphe nucleus, such double-labelled cells represented only a small proportion of the total population of 5-HT-immunoreactive neurons. In the lateral group, the retrograde labelling was mainly unilateral to the injection site but some contralateral labelling was also seen. At caudal levels of the dorsal raphe nucleus, retrogradely labelled cells were observed predominantly in the lateral group. At the level of the dorsolateral tegmental nucleus, few 5-HT of 5-HT/HRP labelled cells were observed in the lateral group, although HRP retrogradely labelled neurons were present. Double-stained cells were detected also in the medial raphe nucleus (corresponding to the B8 cell group according to the nomenclature of Dahlström and Fuxe13), among the fibres of the medial lemniscus (B9), and in nucleus raphe pontis (B5).</div></front></TEI><affiliations><list><country><li>Italie</li><li>Royaume-Uni</li></country><region><li>Angleterre</li><li>Oxfordshire</li></region><settlement><li>Oxford</li></settlement></list><tree><country name="Italie"><noRegion><name sortKey="Consolazione, Adriana" sort="Consolazione, Adriana" uniqKey="Consolazione A" first="Adriana" last="Consolazione">Adriana Consolazione</name></noRegion><name sortKey="Consolazione, Adriana" sort="Consolazione, Adriana" uniqKey="Consolazione A" first="Adriana" last="Consolazione">Adriana Consolazione</name></country><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Priestley, John V" sort="Priestley, John V" uniqKey="Priestley J" first="John V." last="Priestley">John V. Priestley</name></region><name sortKey="Cuello, A Claudio" sort="Cuello, A Claudio" uniqKey="Cuello A" first="A. Claudio" last="Cuello">A. Claudio Cuello</name><name sortKey="Priestley, John V" sort="Priestley, John V" uniqKey="Priestley J" first="John V." last="Priestley">John V. Priestley</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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